Indian Journal of Research in Homeopathy

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 14  |  Issue : 4  |  Page : 251--259

Understanding the role of homoeopathic preparation of Berberis vulgaris in mitigation of sodium oxalate- induced hyperoxaluria: An experimental approach


Bhavani Tamilarasan1, Porkodi Karthikeyan1, Pugazhendhi Kannan1, Kalaiselvi Periandavan1, Raj K Manchanda2, Anil Khurana2, Debadatta Nayak2, Shanthi Palainivelu3 
1 Department of Medical Biochemistry, Dr ALM Post Graduate Institute for Basic Medical Sciences, University of Madras, Chennai, Tamil Nadu, India
2 Central Council for Research in Homoeopathy, New Delhi, India
3 Pathology, Dr ALM Post Graduate Institute for Basic Medical Sciences, University of Madras, Chennai, Tamil Nadu, India

Correspondence Address:
Dr. Kalaiselvi Periandavan
Department of Medical Biochemistry, Dr ALM Post Graduate Institute for Basic Medical Sciences, University of Madras, Chennai, Tamil Nadu
India

Background: Hyperoxaluria and calcium oxalate crystal deposition in the kidneys lead to overproduction of reactive oxygen species, resulting in the development of oxidative stress and renal injury. At the cellular levels, mitochondria and NADPH oxidase involved in reactive oxygen species production play a crucial role in the pathogenesis of hyperoxaluria-induced renal injury. Objective: The objective was to investigate the therapeutical effect of homoeopathic drug Berberis vulgaris, a potent antioxidant, upon regulation of NADPH oxidase against acute high-grade sodium oxalate-induced hyperoxaluria in rats. Materials and Methods: Hyperoxaluria was induced in male Wistar rats by administering a single dose of sodium oxalate (70 mg/kg body weight) intraperitoneally, and the treatment groups were pre-treated with homoeopathic drug Berberis vulgaris 6c (20 μl/100 g of body weight) ultra low dose for 7 days. Results: Berberis vulgaris significantly reduces hyperoxaluria-induced oxidative stress and restores antioxidant enzyme activities in kidney tissue. Histological analysis depicted that Berberis vulgaris treatment decreases renal epithelial damage and inflammation and restored normal glomerular morphology. Furthermore, immunoblotting analyses of NADPH oxidase revealed significant increased activity in the renal tissue of hyperoxaluric rats when compared to that of control rats. This has been brought back to normal by Berberis vulgaris treatment. Conclusion: Thus, our results emphasised that the Homoeopathy drug Berberis vulgaris has been effective in ameliorating sodium oxalate-induced acute hyperoxaluria in Wistar rats by modulating mitochondrial oxidative stress through the inhibition of NADPH oxidase.


How to cite this article:
Tamilarasan B, Karthikeyan P, Kannan P, Periandavan K, Manchanda RK, Khurana A, Nayak D, Palainivelu S. Understanding the role of homoeopathic preparation of Berberis vulgaris in mitigation of sodium oxalate- induced hyperoxaluria: An experimental approach.Indian J Res Homoeopathy 2020;14:251-259


How to cite this URL:
Tamilarasan B, Karthikeyan P, Kannan P, Periandavan K, Manchanda RK, Khurana A, Nayak D, Palainivelu S. Understanding the role of homoeopathic preparation of Berberis vulgaris in mitigation of sodium oxalate- induced hyperoxaluria: An experimental approach. Indian J Res Homoeopathy [serial online] 2020 [cited 2021 Jan 23 ];14:251-259
Available from: https://www.ijrh.org/article.asp?issn=0974-7168;year=2020;volume=14;issue=4;spage=251;epage=259;aulast=Tamilarasan;type=0