Indian Journal of Research in Homeopathy

ORIGINAL ARTICLE
Year
: 2017  |  Volume : 11  |  Issue : 3  |  Page : 158--169

Anti-inflammatory homoeopathic drug dilutions restrain lipopolysaccharide-induced release of pro-inflammatory cytokines: In vitro and in vivo evidence


Umesh B Mahajan1, Ajit K Walke1, Mahendra V Kardile1, Sameer N Goyal1, Sumit Siddharth2, Chanakya Nath Kundu2, Shreesh Ojha3, Chandragouda R Patil1 
1 Department of Pharmacology, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dhule, Maharashtra, India
2 School of Biotechnology, Cancer Biology Laboratory, KIIT School of Biotechnology, KIIT University, Bhubaneswar, Odisha, India
3 Department of Pharmacology and Therapeutics, College of Medicine and Health Sciences, University, Al-Ain, United Arab Emirates

Correspondence Address:
Chanakya Nath Kundu
School of Biotechnology, Cancer Biology Laboratory, KIIT School of Biotechnology, KIIT University, Bhubaneswar, Odisha
India
Chandragouda R Patil
Department of Pharmacology, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dhule, Maharashtra
India

Context: The lipopolysaccharide (LPS)-induced cytokine release and oxidative stress are validated experimental parameters used to test anti-inflammatory activity. We investigated the effects of homoeopathic mother tinctures, 6 CH, 30 CH and 200 CH dilutions of Arnica montana, Thuja occidentalis and Bryonia alba against LPS (1 μg/ml)-induced cytokine release from RAW-264.7 cells and human whole-blood culture. Materials and Methods: For in vivo evaluations, mice were orally treated with 0.1 ml drug dilutions twice a day for 5 days followed by an intraperitoneal injection of 0.5 mg/kg LPS. After 24 h, the mice were sacrificed and serum levels of pro-inflammatory cytokines and nitric oxide were determined. The extent of oxidative stress was determined in the liver homogenates as contents of reduced glutathione, malondialdehyde, superoxide dismutase and catalase. Results: The tested drug dilutions significantly reduced in vitro LPS-induced release of tumour necrosis factor-α, interleukin-1 (IL-1) and IL-6 from the RAW-264.7 cells and human whole blood culture. Similar suppression of cytokines was evident in mice serum samples. These drugs also protected mice from the LPS-induced oxidative stress in liver tissue. Conclusions: Our findings substantiate the protective effects of Arnica, Thuja and Bryonia homoeopathic dilutions against LPS-induced cytokine elevations and oxidative stress. This study authenticates the claims of anti-inflammatory efficacy of these homoeopathic drugs.


How to cite this article:
Mahajan UB, Walke AK, Kardile MV, Goyal SN, Siddharth S, Kundu CN, Ojha S, Patil CR. Anti-inflammatory homoeopathic drug dilutions restrain lipopolysaccharide-induced release of pro-inflammatory cytokines: In vitro and in vivo evidence.Indian J Res Homoeopathy 2017;11:158-169


How to cite this URL:
Mahajan UB, Walke AK, Kardile MV, Goyal SN, Siddharth S, Kundu CN, Ojha S, Patil CR. Anti-inflammatory homoeopathic drug dilutions restrain lipopolysaccharide-induced release of pro-inflammatory cytokines: In vitro and in vivo evidence. Indian J Res Homoeopathy [serial online] 2017 [cited 2021 May 16 ];11:158-169
Available from: https://www.ijrh.org/article.asp?issn=0974-7168;year=2017;volume=11;issue=3;spage=158;epage=169;aulast=Mahajan;type=0