FUNDAMENTAL RESEARCH

Year : 2008  |  Volume : 2  |  Issue : 1  |  Page : 1-7

Application of HPTLC in the Standardization of a Homoeopathic Mother Tincture of Nux Vomica

DA Shanbhag, Sunita Jayaraman
,

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Source of Support: None, Conflict of Interest: None

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In this study,we have chosen HPTLC (High Performance Thin Layer Chromatography) as a method of analysis to develop a standard procedure based on fingerprinting characteristics for the evaluation of homreopathic formulations. A simple and accurate HPTLC method has been developed for the quantification of brucine,one of the chief active chemical components of Nux vomica (along with strychnine),and the fingerprinting of the in-house mother tincture of Nux vomica, considered here to be a standard, with that of different marketed samples available from manufacturers of homreopathic medicines in India. This HPTLC method was quantitatively evaluated in terms of stability, repeatability, and accuracy.

Keywords: Standardization of homeopathic mother tinctures; HPTLC, as a tool for standardizing homeopathic mother tinctures; brucine, a measurable component of Nux vomica tincture; fingerprinting homeopathic tinctures with HPTLC.