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  Indian J Med Microbiol
 

Figure 1: Sulphur induces tumor apoptosis in NSCLC cells. (A) The number of viable A549 cells following exposure to different potencies (6C, 30C and 200C) of placebo and sulphur at different concentrations (10, 15, 20 and 30 μl/ml) was determined by trypan-blue dye exclusion assay, and the data are represented graphically (*P<0.05, ***P<0.001 when compared with the respective placebo-treated group). (B) Graphical representation showing percentage of cell death of PBMC confirms that sulphur does not induce apoptosis in normal cells (P<0.001 when compared between survival percentages of un-/sulphur-treated PBMCs). The percent cell death was scored by trypan-blue dye-exclusion assay. (C) Time-dependent effect of sulphur 30C (20 μl/ml), in comparison to placebo, on A549 examined at different time intervals (0-48 h). (D) Time-dependent effect of sulphur 30C (20 μl/ml), in comparison to placebo, on PBMC was examined (*P<0.05 and **P<0.001 when compared with respective control/treated groups). (E) The nature of sulphur induced A549 cells was assayed flow cytometrically using Annexin V-FITC/7-AAD double labelling assay. (F) DAPI staining revealed nuclear morphology of apoptotic cells (blebbing and fragmentation) as indicated by arrowheads in sulphur treated sample when visualized under a fluorescence microscope. Bar length in images indicate 20 μm. Values are the mean ± SEM of three independent experiments in each case or representative of a typical experiment

Figure 1: Sulphur induces tumor apoptosis in NSCLC cells. (A) The number of viable A549 cells following exposure to different potencies (6C, 30C and 200C) of placebo and sulphur at different concentrations (10, 15, 20 and 30 μl/ml) was determined by trypan-blue dye exclusion assay, and the data are represented graphically (*P<0.05, ***P<0.001 when compared with the respective placebo-treated group). (B) Graphical representation showing percentage of cell death of PBMC confirms that sulphur does not induce apoptosis in normal cells (P<0.001 when compared between survival percentages of un-/sulphur-treated PBMCs). The percent cell death was scored by trypan-blue dye-exclusion assay. (C) Time-dependent effect of sulphur 30C (20 μl/ml), in comparison to placebo, on A549 examined at different time intervals (0-48 h). (D) Time-dependent effect of sulphur 30C (20 μl/ml), in comparison to placebo, on PBMC was examined (*P<0.05 and **P<0.001 when compared with respective control/treated groups). (E) The nature of sulphur induced A549 cells was assayed flow cytometrically using Annexin V-FITC/7-AAD double labelling assay. (F) DAPI staining revealed nuclear morphology of apoptotic cells (blebbing and fragmentation) as indicated by arrowheads in sulphur treated sample when visualized under a fluorescence microscope. Bar length in images indicate 20 μm. Values are the mean ± SEM of three independent experiments in each case or representative of a typical experiment