Indian Journal of Research in Homeopathy

ORIGINAL ARTICLE
Year
: 2017  |  Volume : 11  |  Issue : 2  |  Page : 109--117

Comparative standardization study for determination of reserpine in Rauwolfia serpentina homoeopathic mother tinctures manufactured by different pharmaceutical industries using HPTLC as a check for quality control


Binit Kumar Dwivedi1, Manoj Kumar1, Anil Khurana2, Bhopal Singh Arya1, Echur Natrajan Sundaram2, Raj K Manchanda2 
1 Dr. DP Rastogi Central Research Institute (H), Noida, India
2 Central Council for Research in Homoeopathy, New Delhi, India

Correspondence Address:
Binit Kumar Dwivedi
DDPR Central Research Institute for Homoeopathy, A-1/1 Sector-24 Noida-UP-201301, New Delhi
India

Background: Rauwolfia serpentina (L.) Benth. ex Kurz (Apocynaceae) (Indian snakeroot), popularly known as Sarpagandha (Sanskrit), is used for the treatment of insanity, fever, snake bites, anxiety and in neuropsychiatric conditions. The antihypertensive actions of Reserpine are a result of its ability to deplete catecholamines (amongst other monoamine neurotransmitters) from peripheral sympathetic nerve endings which are normally involved in controlling heart rate, force of cardiac contraction and peripheral vascular resistance. Objective: Comparative study of Reserpine content in R. serpentina homoeopathic mother tinctures manufactured by different pharmaceutical industries and in-house mother tinctures applying high-performance thin-layer chromatography investigative techniques to facilitate the use of correct species. Materials and Methods: The authentic samples of roots of R. serpentina were supplied by Centre of Medicinal Plants Research in Homoeopathy, Emerald, Tamil Nadu, India. Authentic plant material was used to prepare the mother tincture (as per Homoeopathic Pharmacopoeia of India). Reserpine (C33H40N2O9,M.P. 360°C, purity >99% w/w by high-performance liquid chromatography [HPLC]) was purchased from Sigma-Aldrich as a standard reference. The solvents for the study, namely, ethanol, HPLC water, toluene, ethyl acetate, diethylamine and chloroform were of analytical grade purity (MERCK Ltd.,), used throughout. Results: Five samples of mother tinctures were used for the study, in-house mother tinctures (labelled: D and E) of R. serpentina shows a higher amount of Reserpine content than the marketed samples (labelled: A, B and C). Conclusion: It may be concluded that mother tinctures prepared by authentic plants showed the excess amount of Reserpine rather than that of mother tinctures procured from the market.


How to cite this article:
Dwivedi BK, Kumar M, Khurana A, Arya BS, Sundaram EN, Manchanda RK. Comparative standardization study for determination of reserpine in Rauwolfia serpentina homoeopathic mother tinctures manufactured by different pharmaceutical industries using HPTLC as a check for quality control.Indian J Res Homoeopathy 2017;11:109-117


How to cite this URL:
Dwivedi BK, Kumar M, Khurana A, Arya BS, Sundaram EN, Manchanda RK. Comparative standardization study for determination of reserpine in Rauwolfia serpentina homoeopathic mother tinctures manufactured by different pharmaceutical industries using HPTLC as a check for quality control. Indian J Res Homoeopathy [serial online] 2017 [cited 2020 Sep 24 ];11:109-117
Available from: http://www.ijrh.org/article.asp?issn=0974-7168;year=2017;volume=11;issue=2;spage=109;epage=117;aulast=Dwivedi;type=0