|Year : 2016 | Volume
| Issue : 1 | Page : 36-41
Comparative analysis of antibacterial activity of povidone iodine and homoeopathic mother tinctures as antiseptics
Muhammad Mohsin Zaman1, Muhammad Aslam Shaad1, Saeed Ahmad2, Waheed Mumtaz Abbasi3, Tayyeba Rehman3
1 Department of Biochemistry, Bahauddin Zakariya University, Multan, Pakistan
2 Department of Pharmacy, Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
3 Department of Homeopathic Medical Sciences, University College of Conventional Medicine, Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
|Date of Submission||14-Nov-2015|
|Date of Acceptance||29-Feb-2016|
|Date of Web Publication||21-Mar-2016|
Muhammad Mohsin Zaman
Department of Biochemistry, Bahauddin Zakariya University, Multan
Source of Support: None, Conflict of Interest: None
Objectives: Antiseptics are used extensively in hospitals and other health care centers (clinics) to control the growth of microbes on living tissues. They are essential parts of infection control practices, especially in wound treatment and aid in the prevention of nosocomial infections. This study was done to evaluate and compare the antibacterial effectiveness of povidone iodine and eight commonly used homoeopathic mother tinctures.
Materials and Methods: The present study was conducted in the Faculty of Pharmacy and alternative Medicine, The Islamia University of Bahawalpur. All the selected Homeopathic mother tinctures were purchased by Masood Homeopathic Pharmaceuticals, Pakistan. Prepared PYODINEâ (povidone iodine) solution 10% w/v, batch no. 084F4 used as positive control while 70% ethanol was used as negative control in each test. These tinctures were tested against three clinically important human skin-pathogenic bacteria (Staphylococcus aureus, Streptococcus pyogenes, and Pseudomonas aeruginosa) by disc diffusion method with povidone iodine as positive standard.
Results: Seven out of eight mother tinctures showed more or less antibacterial activity; some of them have high activity than povidone iodine. Rhus glabra had maximum activity (zone of inhibition 32 mm) against Streptococcus pyogenes and Pseudomonas aeruginosa than povidone iodine and among all the tested mother tinctures.
Conclusion: This study confirms the antibacterial activity and more effectiveness of Thuja occidentalis and Rhus glabra mother tinctures than povidone iodine. The other tested mother tinctures also have antibacterial activity against tested bacteria, except Echinacea.
Keywords: Antibacterial, Antiseptics, Homoeopathic mother tinctures, Pyodine
|How to cite this article:|
Zaman MM, Shaad MA, Ahmad S, Abbasi WM, Rehman T. Comparative analysis of antibacterial activity of povidone iodine and homoeopathic mother tinctures as antiseptics. Indian J Res Homoeopathy 2016;10:36-41
|How to cite this URL:|
Zaman MM, Shaad MA, Ahmad S, Abbasi WM, Rehman T. Comparative analysis of antibacterial activity of povidone iodine and homoeopathic mother tinctures as antiseptics. Indian J Res Homoeopathy [serial online] 2016 [cited 2019 Mar 22];10:36-41. Available from: http://www.ijrh.org/text.asp?2016/10/1/36/179151
| Introduction|| |
Skin and soft tissue infections (SSTIs) are conditions produce considerable morbidity and affect life quality. Every year millions of people around the world experience SSTIs of both acute and chronic nature. A fascinating way of treating superficial skin infections or wounds is through the use of topical antimicrobials or antiseptics.
Antiseptics can be applied to kill or inhibit microorganisms' growth and have the ability to target multiple microbes. However, a common problem is to select the appropriate antiseptics because different pathogens respond in different way to different antiseptics. Increasing bacterial resistance to antibiotics makes the management of superficial skin infections a major medical challenge. Antiseptics have broader spectrums of antimicrobial activity and a reduced potential for selection of bacterial resistance, relative to antibiotics. Therefore, antiseptics are proper alternatives to antibiotics for the prevention and treatment of superficial skin infections. From widely used antiseptics, povidone iodine has a particularly broad spectrum of antimicrobial activity that includes Gram-positive and Gram-negative bacteria, bacterial spores, fungi, protozoa, and viruses. Pyodine is used commonly in preoperative preparations, postoperative cleansing, open wounds, and cuts dressings.
Mother tinctures play a role in homoeopathic prescribing particularly for their organotrophic effects. Homoeopathic mother tinctures are commonly used by homoeopathic physicians at their clinics for various skin infections, chronic ulcerative conditions, and wound infections as topical antiseptics. Homoeopathic tinctures are not antiseptic but bacteriostatic; they do not permit bacteria to flourish in their presence. They do not destroy epithelial tissues and the white blood cells which are the body's first line of defense against infection. Wound treated homoeopathically at the outset rarely become infected, and clinical observations demonstrate that healing is clean and occurs nearly 50% more rapidly than under traditional allopathic or orthodox approaches.
The following homoeopathic plant mother tinctures were selected for the antibacterial testing.
Arnica montana from the family Asteraceae mostly used for bruises, sprains, and muscles pain., It improves the local blood supply and speeds up the healing. It is anti-inflammatory and helps to increase the rate of reabsorption of internal bleeding.
Baptisia tinctoria, a plant of Leguminosae family, is a strong antiseptic and immune stimulant. It is considered effective in infected nipples and infected skin particularly. A study showed moderate antibacterial activity of B. tinctoria and Berberis vulgaris against Staphylococcus aureus.
Berberis aquifolium and Berberis vulgaris being part of Berberidaceae family have a considerable reputation as an antiseptic, blood purifier, and tissue cleanser and mostly used for skin diseases such as psoriasis, eczema, acne, and cold sores.
Calendula officinalis is the most frequently used plant in various homoeopathic and herbal topical applications such as ointments, lotions, sprays, and dressings in cases of different skin problems such as injuries, lacerations, wounds, and ulcers in various studies and research works; Calendula is a great wound healer, antiseptic, and tissue proliferator.,
Echinacea angustifolia is a medical immune enhancer for skin diseases and general infections. It is valued by North American Indians of the USA. It became famed for cleaning and healing suppurative wounds. Homoeopathically, it is used in cases of blood poisoning and septic conditions, also in cases of recurring boils, erysipelas, malignant ulcers, and gangrene. However, a study showed that Echinacea angustifolia has no antibacterial effect against various human pathogenic bacteria.
Rhus glabra member of Anacardiaceae family is known for its use in various skin eruptions and putrescent conditions with tendency to ulceration.
Thuja occidentalis belongs to Cupressaceae family. It is used in ulcers, fig warts, boils, and chilblains;, also in cases of polyps, epithelioma, tubercles, carbuncles, and ulcers especially at the anorectal region; and also in skin eruptions only at the covered parts. The present study is an attempt to evaluate and compare the antibacterial activity of above-mentioned homoeopathic plant mother tinctures with povidone iodine.
| Materials and Methods|| |
The present study was conducted in the Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur. All the selected homoeopathic mother tinctures were purchased by Masood Homoeopathic Pharmaceuticals, Pakistan. Prepared PYODINE® (povidone iodine) solution 10% w/v, batch no. 084F4 was used as positive control while 70% ethanol was used as negative control in each test.
Preparation of Inoculum
Primary culture plate of the selected three bacteria was made by streaking the swab of the KWIK-STICK containing the respective bacterial strain on the fresh sterilized nutrient agar plate. A few colonies from the overnight primary culture plate of Streptococcus pyogenes, Staphylococcus aureus, and Pseudomonas aeruginosa were suspended in 10 ml sterile nutrient broth separately for three bacteria, and the solution was adjusted to the 0.5 McFarland equivalence turbidity standard.
Antibacterial Susceptibility Test
All the selected homoeopathic mother tinctures along with povidone iodine were screened against three pathogenic bacterial strains. The tested organisms were Staphylococcus aureus ATCC-6538, Streptococcus pyogenes ATCC-19615, and Pseudomonas aeruginosa ATCC-9027 obtained from Microbiologics Inc., Minnesota, USA.
For testing the antibacterial activity, the disk diffusion method was used. Nutrient agar plates were made by pouring (15 ml) sterilized nutrient agar medium and allowed to solidify for a few minutes in aseptic condition. A volume of 60 µl of prepared inoculum was poured into each plate. With the help of L-shaped glass rod spreader, the poured inoculum is spread on the whole surface of the agar uniformly. Filter paper discs of 6 mm diameter loaded with 10 µl of one selected mother tincture, povidone iodine and 70% ethanol separately were placed on the surface of the bacteria seeded nutrient agar medium in the respective labeled area. All the plates were incubated in an inverted position for 24 h at 37°C. After incubation, the clear zone surrounding each disc (zone of inhibition) was measured with the help of Vernier caliper. The work performed in triplicate for each mother tincture against each bacterial strain.
Analysis of variance was applied for statistical analysis of results. P < 0.05 was considered to be statistically significant.
| Results and Discussion|| |
[Table 1] shows the broad antibacterial activity of povidone iodine against all the three bacteria ranging 20–25 mm and had a maximum activity against Staphylococcus aureus while also effective against Streptococcus pyogenes and Pseudomonas aeruginosa. Rhus glabra had maximum zone of inhibition (32 mm) against Streptococcus pyogenes and Pseudomonas aeruginosa than pyodine and had also higher activity among all the tested mother tincture against Pseudomonas aeruginosa. [Figure 1] Thuja occidentalis was found to be most active than pyodine against Streptococcus pyogenes and Pseudomonas aeruginosa [Figure 2] and had also higher activity among all the tested mother tincture against Streptococcus pyogenes. Calendula officinalis was found to be more active than pyodine against Streptococcus pyogenes (20.6 mm) [Figure 3] while Berberis vulgaris had maximum activity among all the mother tinctures against Staphylococcus aureus (21.3 mm) [Figure 4]. Arnica showed moderate antibacterial activity (15.3 mm) against Streptococcus pyogenes and Staphylococcus aureus while showed 9 mm zone of inhibition against Pseudomonas aeruginosa. Baptisia showed 10.3 mm zone on inhibitions against Streptococcus pyogenes and Staphylococcus aureus while showed 9 mm zone of inhibition against Pseudomonas aeruginosa. Berberis aquifolium had also moderate activity against all tested bacteria with zone of inhibitions of 14.3 mm, 6.3 mm, and 7.3 mm against Staphylococcus aureus, Streptococcus pyogenes, and Pseudomonas aeruginosa, respectively. Echinacea angustifolia had no activity against any bacteria and the result was in line with another study that also showed no antibacterial activity against pathogenic bacteria. The diameters of growth of inhibitions of different mother tincture were 32.3 mm. Povidone iodine showed diameters of inhibitions in a range of 20–24 mm. There was statistically significant difference between the zone of inhibition values of povidone iodine and mother tinctures (P< 0.05) [Figure 5], [Figure 6], [Figure 7].
|Table 1: Results of mother tinctures, Povidone iodine and alcohol against different bacterial strains|
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|Figure 1: Antibacterial activity of Rhus glabra against Pseudomonas aeruginosa. A = alcohol, Pyo = pyodine, R = Rhus glabra, P.A = Pseudomonas aeruginosa|
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|Figure 2: Antibacterial activity of Thuja occidentalis against Pseudomonas aeruginosa. A = alcohol, Pyo = pyodine, T= Thuja occidentalis, P.A= Pseudomonas aeruginosa|
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|Figure 3: Antibacterial activity of Calendula officinalis against Streptococcus pyogenes. A = alcohol, Pyo = pyodine, C = Calendula, S.P = Streptococcus pyogenes|
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|Figure 4: Antibacterial activity of Berberis vulgaris against Staphylococcus aureus. A = alcohol, P = pyodine, Be = Berberis vulgaris, S.A = Staphylococcus aureus|
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|Figure 5: Comparison of homoeopathic mother tinctures with povidone iodine against Pseudomonas aeruginosa. Values are expressed as mean (n = 3). Diameter of disk was 6 mm|
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|Figure 6: Comparison of homoeopathic mother tinctures with povidone iodine against Staphylococcus aureus. Values are expressed as mean (n = 3). Diameter of disk was 6 mm|
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|Figure 7: Comparison of homoeopathic mother tinctures with povidone iodine against Streptococcus pyogenes. Values are expressed as mean (n = 3). Diameter of disk was 6 mm|
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| Conclusion|| |
Rhus glabra and Thuja occidentalis (against two bacterial strains) and Calendula officinalis (against one bacterial strain) exhibited higher efficacy than pyodine. These three homoeopathic mother tinctures showed excellent zone of inhibition and were found to be more effective than pyodine, while A. montana, B. tinctoria, Berberis vulgaris, and Berberis aquifolium were found to be moderately effective against all the bacteria tested. Only Echinacea angustifolia showed no activity against any of the three bacteria. Hence, most of the homoeopathic mother tinctures proved to be effective in inhibiting the in vitro growth of three clinically important skin-pathogenic bacteria, Staphylococcus aureus, S. pyogenes, and P. aeruginosa.
Financial Support and Sponsorship
Conflicts of Interest
There are no conflicts of interest.
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[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6], [Figure 7]
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