Dose-dependent effect of homoeopathic drug Zinc sulphate on plant growth using Bacopa monnieri as model system
Vivek Kumar Gupta1, Jutika Rani Ray1, Vishal Kumar Singh1, Surya Deo Pathak2, Chaturbhuja Nayak3, Mahendra P Darokar1
1 Molecular Bioprospection Department, Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Lucknow, Uttar Pradesh, India
2 Homoeopathic Drug Research Institute, Viraj Khand, Lucknow, Uttar Pradesh, India
3 Former Director General, Central Council for Research in Homoeopathy, Ministry of Health and Family Welfare, New Delhi, India
Mahendra P Darokar
Department of Molecular Bioprospection, Central Institute of Medicinal and Aromatic Plants, Kukrailpicnic Spot Road, Lucknow - 226 015, Uttar Pradesh
Source of Support: Central Council for Research in Homoeopathy, Department of AYUSH, Ministry of Health and Family Welfare, Govt. of India, New Delhi,, Conflict of Interest: None
Clinical trial registration not applicable
Background: Zinc is one of the essential micronutrients in plants required in very low quantity for plant growth and development. In higher concentration, it is known to to reduce the rate of photosynthesis, So homoeopathic preparations tested to see it role on plan growth.
Objective: To analyse the effect of homoeopathic preparation of Zinc sulphate on plants through in-vitro assay using Bacopa monnieri as a model plant system.
Materials and Methods: Six homoeopathic potencies (1X to 6X) of Zinc sulphate were used on a decimal scale along with the control (MS basal agar medium). The samples were evaluated by adding fixed amount (100 μl) in the media as well as by dipping the explants in the test sample overnight. At the completion of the incubation period (14 days) the fresh and dry weight, number and length of the roots, number and length of the shoots and the number of leaves were analysed.
Results: It was observed that Zinc sulphate showed growth inhibition at potencies from 1X to 5X, whereas at potency 6X, it exhibited growth promotion effect, when compared with the control.
Conclusion: Homoeopathic drug (Zinc sulphate) exhibited growth promotion at higher potency (6X) and growth inhibition at lower potencies (1X to 5X) on Bacopa monneiri.